X-ray fluorescence analysis of iron and manganese distribution in primary dopaminergic neurons
Identifieur interne : 001051 ( Main/Exploration ); précédent : 001050; suivant : 001052X-ray fluorescence analysis of iron and manganese distribution in primary dopaminergic neurons
Auteurs : Tanja Du I [Allemagne] ; Elisabeth Barski [Allemagne] ; Murielle Salome [France] ; Jan C. Koch [Allemagne] ; Mathias B Hr [Allemagne] ; Paul Lingor [Allemagne]Source :
- Journal of Neurochemistry [ 0022-3042 ] ; 2012.
Abstract
Transition metals have been suggested to play a pivotal role in the pathogenesis of Parkinson's disease. X-ray microscopy combined with a cryogenic setup is a powerful method for elemental imaging in low concentrations and high resolution in intact cells, eliminating the need for fixation and sectioning of the specimen. Here, we performed an elemental distribution analysis in cultured primary midbrain neurons with a step size in the order of 300 nm and ∼ 0.1 ppm sensitivity under cryo conditions by using X-ray fluorescence microscopy. We report the elemental mappings on the subcellular level in primary mouse dopaminergic (DAergic) and non-DAergic neurons after treatment with transition metals. Application of Fe2+ resulted in largely extracellular accumulation of iron without preference for the neuronal transmitter subtype. A quantification of different Fe oxidation states was performed using X-ray absorption near edge structure analysis. After treatment with Mn2+, a cytoplasmic/paranuclear localization of Mn was observed preferentially in DAergic neurons, while no prominent signal was detectable after Mn3+ treatment. Immunocytochemical analysis correlated the preferential Mn uptake to increased expression of voltage-gated calcium channels in DAergic neurons. We discuss the implications of this differential elemental distribution for the selective vulnerability of DAergic neurons and Parkinson's disease pathogenesis.
Url:
DOI: 10.1111/jnc.12073
PubMed: 23106162
PubMed Central: 3563009
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000771
- to stream Pmc, to step Curation: 000768
- to stream Pmc, to step Checkpoint: 000554
- to stream Ncbi, to step Merge: 001002
- to stream Ncbi, to step Curation: 001002
- to stream Ncbi, to step Checkpoint: 001002
- to stream Main, to step Merge: 001085
- to stream Main, to step Curation: 001051
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">X-ray fluorescence analysis of iron and manganese distribution in primary dopaminergic neurons</title><author><name sortKey="Du I, Tanja" sort="Du I, Tanja" uniqKey="Du I T" first="Tanja" last="Du I">Tanja Du I</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>The HASYLAB – DESY</institution><addr-line>Hamburg, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Hamburg</wicri:regionArea></affiliation></author><author><name sortKey="Barski, Elisabeth" sort="Barski, Elisabeth" uniqKey="Barski E" first="Elisabeth" last="Barski">Elisabeth Barski</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Neurology, University Medicine Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation></author><author><name sortKey="Salome, Murielle" sort="Salome, Murielle" uniqKey="Salome M" first="Murielle" last="Salome">Murielle Salome</name><affiliation wicri:level="1"><nlm:aff id="au3"><institution>European Synchrotron Radiation Facility (ESRF)</institution><addr-line>Grenoble, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Grenoble</wicri:regionArea></affiliation></author><author><name sortKey="Koch, Jan C" sort="Koch, Jan C" uniqKey="Koch J" first="Jan C" last="Koch">Jan C. Koch</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Neurology, University Medicine Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation></author><author><name sortKey="B Hr, Mathias" sort="B Hr, Mathias" uniqKey="B Hr M" first="Mathias" last="B Hr">Mathias B Hr</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Neurology, University Medicine Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au4"><institution>Cluster of Excellence “Nanoscale Microscopy and Molecular Physiology of the Brain”</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation></author><author><name sortKey="Lingor, Paul" sort="Lingor, Paul" uniqKey="Lingor P" first="Paul" last="Lingor">Paul Lingor</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Neurology, University Medicine Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au4"><institution>Cluster of Excellence “Nanoscale Microscopy and Molecular Physiology of the Brain”</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23106162</idno><idno type="pmc">3563009</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563009</idno><idno type="RBID">PMC:3563009</idno><idno type="doi">10.1111/jnc.12073</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000771</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000771</idno><idno type="wicri:Area/Pmc/Curation">000768</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000768</idno><idno type="wicri:Area/Pmc/Checkpoint">000554</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000554</idno><idno type="wicri:Area/Ncbi/Merge">001002</idno><idno type="wicri:Area/Ncbi/Curation">001002</idno><idno type="wicri:Area/Ncbi/Checkpoint">001002</idno><idno type="wicri:doubleKey">0022-3042:2012:Du I T:x:ray:fluorescence</idno><idno type="wicri:Area/Main/Merge">001085</idno><idno type="wicri:Area/Main/Curation">001051</idno><idno type="wicri:Area/Main/Exploration">001051</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">X-ray fluorescence analysis of iron and manganese distribution in primary dopaminergic neurons</title><author><name sortKey="Du I, Tanja" sort="Du I, Tanja" uniqKey="Du I T" first="Tanja" last="Du I">Tanja Du I</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>The HASYLAB – DESY</institution><addr-line>Hamburg, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Hamburg</wicri:regionArea></affiliation></author><author><name sortKey="Barski, Elisabeth" sort="Barski, Elisabeth" uniqKey="Barski E" first="Elisabeth" last="Barski">Elisabeth Barski</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Neurology, University Medicine Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation></author><author><name sortKey="Salome, Murielle" sort="Salome, Murielle" uniqKey="Salome M" first="Murielle" last="Salome">Murielle Salome</name><affiliation wicri:level="1"><nlm:aff id="au3"><institution>European Synchrotron Radiation Facility (ESRF)</institution><addr-line>Grenoble, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Grenoble</wicri:regionArea></affiliation></author><author><name sortKey="Koch, Jan C" sort="Koch, Jan C" uniqKey="Koch J" first="Jan C" last="Koch">Jan C. Koch</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Neurology, University Medicine Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation></author><author><name sortKey="B Hr, Mathias" sort="B Hr, Mathias" uniqKey="B Hr M" first="Mathias" last="B Hr">Mathias B Hr</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Neurology, University Medicine Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au4"><institution>Cluster of Excellence “Nanoscale Microscopy and Molecular Physiology of the Brain”</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation></author><author><name sortKey="Lingor, Paul" sort="Lingor, Paul" uniqKey="Lingor P" first="Paul" last="Lingor">Paul Lingor</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Neurology, University Medicine Göttingen</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au4"><institution>Cluster of Excellence “Nanoscale Microscopy and Molecular Physiology of the Brain”</institution><addr-line>Göttingen, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Göttingen</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Neurochemistry</title><idno type="ISSN">0022-3042</idno><idno type="eISSN">1471-4159</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Transition metals have been suggested to play a pivotal role in the pathogenesis of Parkinson's disease. X-ray microscopy combined with a cryogenic setup is a powerful method for elemental imaging in low concentrations and high resolution in intact cells, eliminating the need for fixation and sectioning of the specimen. Here, we performed an elemental distribution analysis in cultured primary midbrain neurons with a step size in the order of 300 nm and ∼ 0.1 ppm sensitivity under cryo conditions by using X-ray fluorescence microscopy. We report the elemental mappings on the subcellular level in primary mouse dopaminergic (DAergic) and non-DAergic neurons after treatment with transition metals. Application of Fe<sup>2+</sup> resulted in largely extracellular accumulation of iron without preference for the neuronal transmitter subtype. A quantification of different Fe oxidation states was performed using X-ray absorption near edge structure analysis. After treatment with Mn<sup>2+</sup>, a cytoplasmic/paranuclear localization of Mn was observed preferentially in DAergic neurons, while no prominent signal was detectable after Mn<sup>3+</sup> treatment. Immunocytochemical analysis correlated the preferential Mn uptake to increased expression of voltage-gated calcium channels in DAergic neurons. We discuss the implications of this differential elemental distribution for the selective vulnerability of DAergic neurons and Parkinson's disease pathogenesis.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bacquart, T" uniqKey="Bacquart T">T Bacquart</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benedetto, A" uniqKey="Benedetto A">A Benedetto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berg, D" uniqKey="Berg D">D Berg</name></author><author><name sortKey="Hochstrasser, H" uniqKey="Hochstrasser H">H Hochstrasser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Braak" uniqKey="Braak">Braak</name></author><author><name sortKey="H" uniqKey="H">H</name></author><author><name sortKey="Del Tredici" uniqKey="Del Tredici">Del Tredici</name></author><author><name sortKey="K" uniqKey="K">K</name></author><author><name sortKey="Rub" uniqKey="Rub">Rüb</name></author><author><name sortKey="U" uniqKey="U">U</name></author><author><name sortKey="De Vos" uniqKey="De Vos">de Vos</name></author><author><name sortKey="R A" uniqKey="R A">R. A</name></author><author><name sortKey="Jansen Steur" uniqKey="Jansen Steur">Jansen Steur</name></author><author><name sortKey="E N" uniqKey="E N">E. N</name></author><author><name sortKey="Braak" uniqKey="Braak">Braak</name></author><author><name sortKey="E" uniqKey="E">E</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carmona, A" uniqKey="Carmona A">A Carmona</name></author><author><name sortKey="Deves, G" uniqKey="Deves G">G Devès</name></author><author><name sortKey="Ortega, R" uniqKey="Ortega R">R Ortega</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chew, Kcm" uniqKey="Chew K">KCM Chew</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cotte, M" uniqKey="Cotte M">M Cotte</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Covy, Jp" uniqKey="Covy J">JP Covy</name></author><author><name sortKey="Giasson, Bi" uniqKey="Giasson B">BI Giasson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Crossgrove, Js" uniqKey="Crossgrove J">JS Crossgrove</name></author><author><name sortKey="Yokel, Ra" uniqKey="Yokel R">RA Yokel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Crossgrove, Js" uniqKey="Crossgrove J">JS Crossgrove</name></author><author><name sortKey="Yokel, Ra" uniqKey="Yokel R">RA Yokel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duci, T" uniqKey="Duci T">T Ducić</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Erikson, Km" uniqKey="Erikson K">KM Erikson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eriksson, H" uniqKey="Eriksson H">H Eriksson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fasano, M" uniqKey="Fasano M">M Fasano</name></author><author><name sortKey="Bergamasco, B" uniqKey="Bergamasco B">B Bergamasco</name></author><author><name sortKey="Lopiano, L" uniqKey="Lopiano L">L Lopiano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gaasch, Ja" uniqKey="Gaasch J">JA Gaasch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guilarte, Tr" uniqKey="Guilarte T">TR Guilarte</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamai, D" uniqKey="Hamai D">D HaMai</name></author><author><name sortKey="Campbell, A" uniqKey="Campbell A">A Campbell</name></author><author><name sortKey="Bondy, Sc" uniqKey="Bondy S">SC Bondy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harrison, Pm" uniqKey="Harrison P">PM Harrison</name></author><author><name sortKey="Arosio, P" uniqKey="Arosio P">P Arosio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ide Ektessabi, A" uniqKey="Ide Ektessabi A">A Ide-Ektessabi</name></author><author><name sortKey="Rabionet, M" uniqKey="Rabionet M">M Rabionet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jomova, K" uniqKey="Jomova K">K Jomova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalia, K" uniqKey="Kalia K">K Kalia</name></author><author><name sortKey="Jiang, W" uniqKey="Jiang W">W Jiang</name></author><author><name sortKey="Zheng, W" uniqKey="Zheng W">W Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kostka, M" uniqKey="Kostka M">M Kostka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lingor, P" uniqKey="Lingor P">P Lingor</name></author><author><name sortKey="Unsicker, K" uniqKey="Unsicker K">K Unsicker</name></author><author><name sortKey="Krieglstein, K" uniqKey="Krieglstein K">K Krieglstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matsushita, N" uniqKey="Matsushita N">N Matsushita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mertz, Lm" uniqKey="Mertz L">LM Mertz</name></author><author><name sortKey="Baum, Bj" uniqKey="Baum B">BJ Baum</name></author><author><name sortKey="Ambudkar, Is" uniqKey="Ambudkar I">IS Ambudkar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Migheli, R" uniqKey="Migheli R">R Migheli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moos, T" uniqKey="Moos T">T Moos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olanow, Cw" uniqKey="Olanow C">CW Olanow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ortega, R" uniqKey="Ortega R">R Ortega</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ortega, R" uniqKey="Ortega R">R Ortega</name></author><author><name sortKey="Deves, G" uniqKey="Deves G">G Devès</name></author><author><name sortKey="Carmona, A" uniqKey="Carmona A">A Carmona</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Papanikolaou, G" uniqKey="Papanikolaou G">G Papanikolaou</name></author><author><name sortKey="Pantopoulos, K" uniqKey="Pantopoulos K">K Pantopoulos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paunesku, T" uniqKey="Paunesku T">T Paunesku</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qin, Z" uniqKey="Qin Z">Z Qin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salazar, J" uniqKey="Salazar J">J Salazar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shimoda, K" uniqKey="Shimoda K">K Shimoda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sikk, K" uniqKey="Sikk K">K Sikk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Strickland, D" uniqKey="Strickland D">D Strickland</name></author><author><name sortKey="Bertoni, Jm" uniqKey="Bertoni J">JM Bertoni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Szczerbowska Boruchowska, M" uniqKey="Szczerbowska Boruchowska M">M Szczerbowska-Boruchowska</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uversky, Vn" uniqKey="Uversky V">VN Uversky</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Fink, Al" uniqKey="Fink A">AL Fink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yokel, Ra" uniqKey="Yokel R">RA Yokel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zecca, L" uniqKey="Zecca L">L Zecca</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li><li>France</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Du I, Tanja" sort="Du I, Tanja" uniqKey="Du I T" first="Tanja" last="Du I">Tanja Du I</name></noRegion><name sortKey="B Hr, Mathias" sort="B Hr, Mathias" uniqKey="B Hr M" first="Mathias" last="B Hr">Mathias B Hr</name><name sortKey="B Hr, Mathias" sort="B Hr, Mathias" uniqKey="B Hr M" first="Mathias" last="B Hr">Mathias B Hr</name><name sortKey="Barski, Elisabeth" sort="Barski, Elisabeth" uniqKey="Barski E" first="Elisabeth" last="Barski">Elisabeth Barski</name><name sortKey="Koch, Jan C" sort="Koch, Jan C" uniqKey="Koch J" first="Jan C" last="Koch">Jan C. Koch</name><name sortKey="Lingor, Paul" sort="Lingor, Paul" uniqKey="Lingor P" first="Paul" last="Lingor">Paul Lingor</name><name sortKey="Lingor, Paul" sort="Lingor, Paul" uniqKey="Lingor P" first="Paul" last="Lingor">Paul Lingor</name></country><country name="France"><noRegion><name sortKey="Salome, Murielle" sort="Salome, Murielle" uniqKey="Salome M" first="Murielle" last="Salome">Murielle Salome</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001051 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001051 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonFranceV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3563009
|texte= X-ray fluorescence analysis of iron and manganese distribution in primary dopaminergic neurons
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23106162" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ParkinsonFranceV1
| This area was generated with Dilib version V0.6.29. |  |